Abstract
DNA molecular self-assembly technology can be used to build different nanostructures from one dimension to three dimension, and these structures have many potential uses in micro/nano-electronics, nano-biology as well as other fields. We have successfully assembled one dimension DNA nanotubes by using double-crossover (DX) DNA tiles. The nanotube structures were characterized by atomic force microscope (AFM), native-PAGE gel electrophoresis, fluorescence microscope and transmission electron microscope (TEM). The results show that the diameter of these DNA nanotubes are between 7 and 20 nm, and the longest diameter can reach to more than 50 μm. To stabilize these nanotubes, each strand was phosphorylated chemically on the 5'end. After assembling the nanotubes, T4 DNA ligase was used to ligate the nicks. AFM detection indicates that the ligated DNA nanotubes can keep intact tubular structure better than the unligated one, which reveals that the ligation method can strengthen the DNA nanotubes. The robust nanostructures would promote the research of DNA nanotubes in applications of micro/nano-technology.
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