Abstract
A main active component cordycepin was extracted and purified from cultured Cordyceps militaris. The crude extract was microwave extracted and then purified using a cation exchange resin (CER) of LSD-001. The adsorption ability of the resin was investigated using equilibrium adsorption isotherm including Langmuir and Freundlich model, and the equilibrated relationship between cordycepin and LSD-001 could be well described by Langmuir equation. The influential factors of desorption experiments, such as NH3 concentration, pH value and ethanol content of desorption solution, along with desorption time and temperature were successively investigated. The optimal desorption conditions were confirmed as: 0.2 mol L-1 NH3 combined with 80% ethanol (v/v), desorbed for 2 h at 25 °C and pH 14. Compared with the content in crude C. militaris (2.5 mg g-1), cordycepin in the final purified products (144.6 mg•g-1) was increased 58-fold after one cycle of dynamic adsorption and desorption on resin LSD-001.
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