Preoperative Prediction of BRAF Mutation Status in Colorectal Cancer Using a Clinical-radiomics Model

Abstract

This study aimed to develop a clinically practical model to predict V-raf murine sarcoma viral oncogene homolog B1 (BRAF) mutation in colorectal cancer according to radiomic signatures based on computed tomography (CT) and clinical risk factors, and to determine the model's diagnostic accuracy for BRAF mutation status. This retrospective study included 140 patients with colorectal cancer. The significant clinical risk factors were used to build the clinical model; the least absolute shrinkage and selection operator algorithm was adopted to construct a radiomics signature according to imaging features of the tumor lesion, and stepwise logistic regression was applied to select the significant variables to develop the clinical-radiomics model. The predictive performance was evaluated by receiver operating characteristic curve analysis, calibration curve analysis, and decision curve analysis. The radscore, generated by 5 selected radiomics features, demonstrated a favorable ability to predict BRAF mutation in both the training (area under the receiver operating characteristic curve [AUC] 0.93) and validation (AUC 0.87) cohorts. Subsequently, integrating two independent predictors (including the radscore and clinical risk factors) into a nomogram exhibited more favorable discriminatory performance, with the AUC improved to 0.95 and 0.88 in both cohorts. Moreover, the accuracy for predicting BRAF mutations was higher than that of the clinical model, ranging from 0.70 to 0.89. The proposed CT-based radiomics signature is associated with BRAF mutations. The present study also proposes a combined model can potentially be applied in the individual preoperative prediction of BRAF mutation status in colorectal cancer. CT-based radiomics showed satisfactory diagnostic significance for the BRAF status in colorectal cancer, the clinical-combined model may be applied in the individual preoperative prediction of BRAF mutation.