Preliminary study on the relationship between the blood supply of rabbit liver VX2 tumor models and the HIFU

Abstract

Objectives To explore the effects of the blood supply extent on the temperature of target site of tumor and volume of coagulation necrosis (V), and to provide experimental evidence for further precise control of dosage of high intensity focused ultrasound (HIFU) and improve the efficiency of HIFU in the treatment. Methods Twenty-four rabbit liver VX2 tumor models were established and were divided into four groups: 10 d group, 15 d group, 20 d group, no blood supply group (rabbits were put to death in the 10 d after the models were established). The same irradiation parameters of HIFU were used to irradiate the hepatic tumor tissue of every group, the real-time temperature of target site of tumor were measured, the software from temperature controller was used to plot the time-temperature curve (TTC), V was measured after HIFU. Residual tumor tissue was resected for pathological observation and microvascular density (MVD) determination. Results ⑴ Histopathological analysis showed that the extent of a tumor's blood supply followed the order 10 d group > 15 d group > 20 d group (P 0.05), Tmax, T1, and k1 between three groups with no blood supply group had statistical difference (P 15 d group > 20 d group > no blood supply group; T2: 10 d group < 15 d group < 20 d group < no blood supply group; ⑷V: 10 d group < 15 d group < 20 d group < no blood supply group. Conclusions The extent of a tumor's blood supply had a significant effect on the temperature-decrease phase but not on the temperature-increase phase during HIFU treatment. The more abundant blood supply of the tumor was, the faster heat abstraction after HIFU was; and the easier the tissue return to normal temperature, the smaller the volume of coagulation necrosis tissue were. Key words: Liver neoplasms, experimental/BS/TH; Ultrasound, high-intensity focused, transrectal; Disease models, animal