Abstract
Several reports have shown antimicrobial and anticancer activities of mucous glycoproteins extracted from the giant African snail Achatina fulica. Anticancer properties of the snail mucous peptides remain incompletely revealed. The aim of this study was to predict anticancer peptides from A. fulica mucus. Two of HPLC-separated mucous fractions (F2 and F5) showed in vitro cytotoxicity against the breast cancer cell line (MCF-7) and normal epithelium cell line (Vero). According to the mass spectrometric analysis, 404 and 424 peptides from the F2 and F5 fractions were identified. Our comprehensive bioinformatics workflow predicted 16 putative cationic and amphipathic anticancer peptides with diverse structures from these two peptidome data. These peptides would be promising molecules for new anti-breast cancer drug development.
Highlights
Breast cancer is one of the most common diseases in women globally [1]
The breast cancer cell line MCF-7 and the kidney epithelial cell line Vero used in this study were kindly provided by the Department of Biochemistry, Faculty of Medicine, Chiangmai University, Thailand and the Genome Institute, National Center for Genetic Engineering and Biotechnology (BIOTEC), Thailand
The A. fulica mucus was successfully collected by intermittent irritation and separated into six fractions by the C18-reverse phase HPLC system using methanol–water mobile phase
Summary
Breast cancer is one of the most common diseases in women globally [1]. Several factors make women at high risk of the breast cancer [2]. Detection and the use of radiation therapy, surgery, and chemotherapeutic drugs including selective estrogen receptor modulators (SERMs) and aromatase inhibitors can reduce invasive breast cancer. The search for target-specific and less side-effect cancer therapy is still undergoing. Anticancer peptides have been proved to be effective small molecules (b 50 amino acids) that can act against cancerous cells by either membranolytic mechanism or disruption of mitochondria [5]. Amphiphilicity levels and hydrophobic arc size allow penetration of these peptides through the cancerous cell membranes and lead to destabilization of the membrane integrity [7,8]. Pleurocidin-like peptides (NRCs) identified from fish could kill breast cancer cells and human mammary epithelial cells by causing membrane
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