Abstract
We have previously shown that ischemic-preconditioning (IP) improves cardiac performance and sarcoplasmic reticular (SR) function in hearts subjected to ischemiareperfusion (IR) or Ca2+-paradox (CP). In this study we examined the effects of IP on the IR or CP induced changes in gene expression for SR proteins in the isolated rat heart. Total RNA was isolated from control as well as IR and CP hearts with and without IP and the mRNA levels for SR Ca’+-pump, Ca’+-release channels and phospholamban were measured. Hearts exposed to IR or CP showed marked depression in the mRNA levels; this decrease in IR or CP hearts was attenuated when either manoeuver was preceded by IP. These results show: 1) SR gene expression is sensitive to brief duration of IR stress, 2) Ca’+-overload may play a major role in alterations observed in the levels of SR genes, and 3) IP attenuates the down regulation of SR gene expression by IR or CP. (Supported by CIHR Group in Experimental Cardiology) ACTIVATION OF PPARa INHIBITS DNA SYNTHESIS IN SMOOTH MUSCLE CELLS Carla Taylor, Natalia Yurkova, Brenda Litchie & Peter Zahradka. Depts. of Foods & Nutrition and Physiology, Univ. of Manitoba and Institute of Cardiovascular Sciences, St. Boniface Research Centre, Winnipeg, MB
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