Abstract
The precipitation of two transport proteins, cytochrome c and hemoglobin, was carried out by using di‐(2‐ethylhexyl) sulfosuccinate, known as aerosol‐OT (AOT), as a precipitating agent. The percent precipitation was 100% when the molar ratio between AOT and the targeted protein was 11 for cytochrome c and 30 for hemoglobin. By using acetone as a polar solvent, the maximum recovery obtained was 98% for cytochrome c and 40% for hemoglobin. The surfactant contamination in the recovered product was below the detection limit. The required usage of surfactant to purify 1 mole of targeted protein was many orders of magnitude smaller than that required for a reverse micellar extraction when using AOT. Advantages of the suggested precipitation method over the reverse micellar extraction method for protein purification are discussed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
