Abstract
In this paper we described an extension of the previously published one-parameter ternary mobile phase optimization to include quaternary mobile phases. Whereas in ternary optimization only mixtures of two from the three isoeluotropic binary phases of water with methanol, tetrahydrofuran and acetonitrile are optimized, with quaternary optimization a blend of all three binary phases is searched for the best separation conditions. This two-parameters optimization formulates first a linear estimate of the retention based on flat planes, fitted through a minimum number of three initial runs. The predicted optimum is verified and an observed differences is used to refine the retention surfaces. After a few such iterations, the true shape is found and the procedure can be stopped. Practical examples were run to demonstrate the possibilities of the procedures.
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