PPARy Expression in Eutopic and Ectopic Endometrium of Reproductive Age Women with Endometriosis

Abstract

Objective: To evaluate the expression of PPARy receptor and to
 compare its expression in eutopic and ectopic endometrium in
 women with endometriosis.
 Method: This is a cross sectional study. Ten female subjects with endometriosis
 that underwent laparoscopy or laparotomy who fulfilled
 the inclusion criteria were recruited by consecutive sampling.
 Two samples were taken, eutopic endometrium and ectopic endometrium
 from endometriosis cyst wall during surgery of each
 subject. PPARy expression was examined by two-step RT-qPCR. Our
 data was statistically examined using the paired t-test and Pearson’s
 correlation test.
 Result: PPARy was found to be expressed in eutopic and ectopic endometrium
 of women with endometriosis using the RT-qPCR method.
 The expression of PPARy was not statistically different in
 eutopic and ectopic endometrium (1.16 relative fold vs 1.25 relative
 fold; p=0.26). By Pearson’s correlation there was a weak positive
 correlation between PPARy expression of eutopic and ectopic endometrium
 (r=0.16).
 Conclusion: PPARy was detected by two-step RT-qPCR in eutopic
 and ectopic endometrium of women with endometriosis. Semiquantification
 of PPARy expression showed that there was no significant
 difference between PPARy expression in eutopic and ectopic endometrium
 of women with endometriosis. There was a weak positive
 correlation between PPARy expression in eutopic and ectopic endometrium
 of women with endometriosis.
 [Indones J Obstet Gynecol 2015; 3-4: 200-205]
 Keywords: endometriosis, PPARy, two-step RT-qPCR