PP51 - Haemodialysis treatment influence on carbonyl stress parameters

Abstract

Chronic renal failure is associated with increased oxidative and carbonyl stress. Even if haemodialysis eliminates toxic metabolic products, the procedure per se is associated with further increased oxidative and carbonyl stress in patients with renal disease. The aim of our work was to evaluate the influence of long term haemodialysis treatment on oxidative and carbonyl stress parameters in patients with chronic renal failure. Methods A total of 58 subjects participated in this study: 16 patients with chronic renal insufficiency (CRF) randomly selected from Carol Davila Hospital patients, 20 patients with end stage renal disease undergoing haemodialysis treatment 3 times weekly (HD) and 22 subjects apparently healthy (C) as controls. On blood samples collected after overnight fasting we have determined the concentrations of Amadori Products (AP), total dicarbonilic compounds (DC), malondialdehyde (MDA) and total thiols (SH). Results AP, MDA and DC were significantly increased in patients with chronic renal failure (CRF) while total thiols levels were significantly decreased compared with the controls. Interestingly, for patients undertaking haemodialysis treatment (HD) the AP levels were not significantly increased compared with controls while SH levels were increased. MDA and DC levels were also significantly increased in HD patients compared with the controls. We have not found any significant correlation between AP levels and DC levels in our subjects. Conclusion Haemodialysis treatment is lowering Amadori Products levels in end stage renal disease patients while having no beneficial effect on total dicarbonilic compounds levels. Increased total thiols levels found in HD patients may constitute an activated antioxidant answer to increased oxidative stress