Abstract
Objective To determine HPV-16 prevalence in patients diagnosed with oral squamous cell carcinoma. Two studies were perfomed; the first one was a retrospective study in which paraffin fixed samples received at Faculty of Dentistry were analized between the period of years 1995–2012 (S1). A second study, fresh samples from The National Institute of Cancer were analized through years 2009–2012 (S2). Materials and Methods In the S1 study, 60 paraffin fixed samples were taken: 30 from squamous cell carcinoma and 30 coming from normal oral mucosa. These samples were collected between the years 1995–2005 at the Faculty of Dentistry, which exclusion criteria was that they were not fixed in buffered formaldehyde and without necrosis. Two 10 μ sections from the above mentioned samples were used and their DNA extracted. Then the DNA went through standard PCR for detection of HPV-16. Negative samples from both sources underwent Beta-globin PCR to determine DNA integrity. In S2, 22 samples were obtained, where the exclusion criteria was that they came from 18 or older patients, without previous oncologic treatment and with a normal physical exam. The samples were processed through real time PCR to detect HPV-16 and Beta-globin to detect DNA integrity. Results In the S1 study, only one sample was detected positive for HPV-16 virus and zero for normal oral mucosa. Beta-globin turned out positive in the 24 samples from squamous cell carcinoma and in 9 of the normal oral mucosa. Zero of the cancer samples were positive for HPV-16 in the S2 study. Conclusions In both studies, HPV-16 presence was not detected in squamous cell carcinoma samples. In the S2 study, HPV-16 was also not detected in samples coming from normal oral mucosa. We cannot find a positive correlation between HPV-16 presence and squamous cell carcinoma nor normal oral mucosa samples.
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