Abstract
DNA typing was performed on 508 unrelated Japanese volunteers using the 15 short tandem repeats (STR) loci, that is, D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, and FGA, present in the PowerPlex™ 16 System. The allele frequencies, heterozygosity, power of discrimination, mean exclusion chance and polymorphism information content of all loci were calculated by statistical analysis. The combined matching probability and the combined mean exclusion chance in Japanese was 1 in 1.81×0 17 and 0.9999989, respectively. None of the 15 loci were found to deviate from Hardy–Weinberg equilibrium (HWE) according to the results of the exact test and the homozygosity test. There was also no evidence for correlation of alleles between loci. This study demonstrates that this multiplex system is a useful and convenient tool for forensic identification and parentage testing in Japan.
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