Powerful benefits of metel (Datura metel L.) amethyst cultivar seed extract nano-chitosan gel for gingival wound healing

Skin, as the largest organ in the human and animal body, serves as the main protection against various external factors such as sharp objects, extreme temperatures, chemicals, or physical trauma. This study aims to evaluate the effectiveness of seaweed (Ulva lactuca) extract-based hydrogel in accelerating incision wound healing in Rattus norvegicus rats. This experimental study used a post-test only controlled group design with six treatment groups: Ulva lactuca hydrogel 5%, 10%, 15%, positive control (Bioplasenton), negative control (wound without treatment), and rat group without treatment. Data were analyzed using ANOVA test. The results showed that Ulva lactuca hydrogel 5% gave the best results in accelerating wound healing. The wound diameter in the 5% group decreased significantly, from 15.3 cm on day 0 to 10.9 cm on day 3, 4.7 cm on day 6, and 2.36 cm on day 14. Histopathology results showed increased epithelialization, decreased inflammation, and increased collagen deposition in the 5% group compared with the control group. Quantitatively, the 5% hydrogel group achieved wound healing up to 30% faster than the positive control and 50% faster than the negative control. The 5% Ulva lactuca hydrogel proved to be most effective in accelerating wound healing by modulating inflammation and promoting tissue regeneration. This concentration provides an optimal balance between efficacy and safety, making it a potential alternative for wound therapy.

Background: The Indonesia Basic Health Research data show the prevalence of wounds including incision/scratches/stab wounds reaches 20.1%. Natural herbal products play an important role in the wound healing process functioning as anti-inflammatories, antioxidants, antimicrobials, and collagen formation. Mango leaves are often considered waste even though they have many health benefits with the high content of coactive compounds, such as alkaloids, flavonoids, tannins, and saponins which are good for cut wound healing. The use of gel is more stable and controlled release compared to other topical preparations. Gedong Gincu mangoes originating from West Java have unique properties and they are marketed widely in some areas including Cirebon. Aims: This study aims to identify the most effective concentration of Gedong Gincu mango leaf extract on cut wound healing in male white rats (Rattus norvegicus) of the Wistar Strain. Methods: This study used a post-test-only control group design. The extract gel used three levels of concentration, namely 40%, 50%, and 60%. The positive control used 10% povidone iodine while the negative control used a gel base. The gel was applied to the cut wound twice a day for 14 days. Results: The average length of the cut wound was 0.916 cm, 0.912 cm, and 1.050 cm for concentration levels of 40%, 50%, and 60%. For the positive and negative controls, it reached 0.996 cm and 0.702 cm respectively. This indicates no significant difference in the length of the cut wound with a p-value of 0.121 (p>0.05). The wound healing process was effective at a concentration level of 60% as indicated by the disappearance of erythema and edema. Conclusion: Mango leaf extract gel (Mangifera indica L.var. Gedong Gincu) is effective in healing cut wounds with the disappearance of erythema and edema in male white rats (Rattus norvegicus) Wistar strain with a concentration of 60%. Received: 25 September 2024 | Reviewed: 19 October 2024 | Revised: 30 November 2024 | Accepted: 15 December 2024.

Burdock root (Arctium lappa L.) is a good plant for wound healing because it contains lignans, flavonoids, and phenolic acid which have the potential as anti-inflammatory, anti-allergic, anti-cancer, free radical scavengers and antioxidants. This research aims to determine the effectiveness of burdock root extract (Arctium lappa L.) on wound healing in white rats (Rattus norvegicus). The research design of laboratory experiments, with animal samples Rattus norvegicus, were grouped into five groups: three treatment groups with burdock root extract concentrations of 15%, 30%, and 45% and two control groups such positive control with povidone iodine and negative control with basic gel. The results showed that burdock root extract (Arctium lappa L.) concentrations of 30% and 45% had significant differences between the positive and negative control groups in the wound healing process. Burdock root extract (Arctium lappa L.) with a concentration of 45% has the best effectiveness compared to a concentration of 30% or 15% in the healing process of cuts in white rats (Rattus norvegicus).

Burns are caused by heat exposure, such as fire, radiation, electricity or chemicals that can damage the skin and affect the body's systems. The aim of this study was to find out the effect of the ethanol extract of Malacca leaves (Phyllanthus emblica) on the number of fibroblast cells in white rats (Rattus norvegicus) that have burned. This study used 24 white rats (Rattus norvegicus) were divided into 4 groups that smeared with aquadest as a negative control (P1), 5% ethanol extract gel of Malacca leaves (P2), 10% ethanol extract gel of Malacca leaves (P3), and positive control applied with bioplasenton® gel (P4). The IIA degree burn were created by placing a 2x2 cm hot iron plate on the back of the rat for 5 seconds. The euthanasia performed to all animal and the skin samples were collected after 15 days of treatment. Then histopathological preparations were made using HE staining. The number of fibroblast cells were analyzed by ANOVA test. The average number of white rats (Rattus norvegicus) fibroblast cells that suffered burns P1 (negative control) had a number of 7 ± 1.4 cells/visual. Whereas th e P2 group had a number of 4.2 ± 1.58 cells/visual. This value has a significant difference with the negative control. But the P2 and P3 values (3 ± 1.51 cells/visual) there is no significant different with the P4 value (positive control) with an average number of P4 fibroblast cells were 2 ± 0.4 cells/visual. The results of this study concluded that the ethanol extract of malacca leaves 5% and 10% had an effect againts accelerating burns healing in white rats ( Rattus norvegicus).

Background: Chitosan has biocompatibility, non-toxic, biodegrade ability, and polyelectrolyte properties. It has been shown to be antimicrobial and accelerate wound healing. Chitosan gel from the waste of white shrimp head can be a new innovation which is effective to inhibit periodonto-pathogenic bacteria and to accelerate wound healing. Aim: To determine the potential of chitosan gel from white shrimp head (Litopenaeus vannamei) waste to inhibit periodonto-pathogenic bacteria and to accelerate wound healing in white mice (Mus musculus). Method: The research was experimental laboratory research. The design of this study was post-test only with control group design. Research on bacterial inhibition was done by 6-team, 5 times repetitions, and 5 treatments;1%, 2%, 3% chitosan gel positive control (Metronidazole disk) and negative control. The subjects of this wound healing research were 24 white mice (Mus musculus) with incisions on the back. They was divided into treatment groups and control groups with glycerol. Clinical and histological observations were carried out on days 1, 3, and 7. Result: The Mann Whitney test results showed a significant difference in inhibition of 1%, 2%, 3% chitosan gel against the Aggregatibacter actinomycetemcomitans bacteria (p

This study aimed to analyze the effects of supportive therapy using a combination of Limosilactobacillus reuteri (L. reuteri) and hyaluronic acid (HA) gel on the wound-healing process during inflammatory, proliferative, and remodeling/maturation phases following tooth extraction.Forty-five white Wistar rats (Rattus norvegicus) were divided into five treatment groups after the left mandibular central incisor was extracted. The experimental groups were treated with HA gel, probiotic L. reuteri gel, a combination of HA gel and L. reuteri gel, and povidone-iodine (PVP-I). The negative control group was treated with sterile tampons. Tissue samples were collected on days 3, 7, and 14 post-tooth extractions and analyzed histologically using hematoxylin-eosin (HE) staining to evaluate neutrophil numbers, neovascularization, and epithelial thickness.Data analysis was performed using IBM SPSS version 29. Inter-rater reliability was tested using the intraclass correlation coefficient (ICC), followed by normality and homogeneity tests. For normally distributed data, parametric hypothesis testing was conducted using one-way ANOVA at a 95% confidence level (p < 0.05). The post hoc least significant difference test was used to observe significant differences between the groups.Significant differences in neutrophil numbers, neovascularization, and re-epithelialization were found between the negative control and combination group (p < 0.05) on days 3, 7, and 14.HA and L. reuteri combination therapy is effective in accelerating wound healing after tooth extraction.

Diabetes mellitus (DM) is a metabolic disease which is becoming the first number of health problem in Indonesia, based on the results of the Basic Health Research of Ministry of Health in 2013. One of the diabetes complications affected by high levels of blood glucose is diabetic ulcers wich 85% the number of cases was overed by amputation as the result of improper handling. Herbal treatments could be an alternative treatment of diabetic ulcers, one of them is binahong plant. The results of previous studies have shown the ability of ethanolic extract of binahong leaf in accelerating wound healing in diabetic rats. This study would be a continued study to test five kinds of binahong leaf fractions (FDB) with gradual solvent polarity in accelerating wound healing in diabetic ulcer. Wound healing parameters observed were percentage of wound healing from the wound diameter contraction. The test begins with ethanolic extract of binahong leaf fractionation to produce fractions of hexane (FHDB), chloroform (FKDB), ethyl acetate (FEADB), and ethanol (FEDB). Water fraction (FADB) was obtained from ethanolic leaf extract residues of binahong leaf extraction. Each fraction activity was tested topically twice daily on dorsal of test animals that created wounds using a punch biopsy 5 mm diameters. Grouping of test animals were divided into 13 groups with normal control group (non diabetes), negative control (diabetes + topical application of biocream®), positive control (diabetes + topical application of madecassol), and 10 diabetes groups with topical application of 5 kinds of binahong leaf fractions with each fractions consist of a concentrations of 5% and 10% with biocream® as a vehicle. Diabetic parameters measured include blood glucose levels (KGD) and weight lost percentage (PB%) in day 1 and 10 during a 10-day treatment. The results showed the influence of KGD in the condition of diabetes on wound healing rats diabetic ulcers which topically treated with binahong leaf fractions. FADB 10% has significant differences potention in wound healing process in diabetic rats macroscopically that accelerates wound contraction compared with negative and positive control groups (p&lt;0,05).

Ulva lactuca L. contains bioactive substances with anti-inflammatory, antibacterial, and antioxidant properties that aid in healing cut wounds. This research was done to ascertain the histopathological parameter’s effects on the wound-healing capacity of gels made from the ethanol extract of U. lactuca (EEUL). The 45 mice were equally divided into five groups: The gel-based control (group I), the positive control (group II), the pain control (group III), and the treatment groups (groups IV and VI), which received EEUL gel at concentrations of 5 and 10%, respectively. On the mice's back, a 1 cm-long incision was made. A one-way ANOVA, post hoc least significant difference (LSD), and/or Mann-Whitney test were used to statistically analyze the data. Reduced wound scores, healing times, and wound length were observed (p 0.05). On the other hand, the macrophage score, the number of blood vessels, the thickness of the epithelium, and the fibroblast count increased (p0.05%). Topical application of 5% or 10% EEUL gel has accelerated wound healing by increasing macrophage scores, blood vessel density, epithelial thickness, and fibroblast counts while decreasing wound description scores and wound length.

Tooth extraction is a common procedure in dentistry that can result in wounds to the soft and hard tissues of the alveolar process, thereby triggering the healing process. The wound healing process is often complicated by several factors, leading to delayed wound healing. The principles of wound healing involve four phases: hemostasis, inflammation, proliferation, and remodeling. This study aimed to determine the effect of black crab (Scylla serrata) chitosan gel on three-dimensional socket response and fibroblast proliferation after tooth extraction in Wistar rats (Rattus norvegicus) . In this study, chitosan material with a degree of deacetylation of 84.9% was used, thus exhibiting good biological properties in the wound healing process. This research is an experimental study with a post-test only with a controlled group design. The sampling technique used was purposive sampling, and the sample size was calculated using the Federer formula. Observations were made by measuring the mesial-distal, lingual-buccal, and socket depths using calipers and the UNC15 probe and observing the number of fibroblasts histologically on Days 1, 3, and 7. The results of the one-way ANOVA and post hoc LSD tests indicate significant outcomes in socket wound closure and fibroblast proliferation in the chitosan gel group. This study demonstrates that black crab (Scylla serrata) chitosan gel is effective in accelerating socket wound closure and stimulating fibroblasts in socket wounds after tooth extraction. Keywords: tooth extraction, black crab chitosan gel, socket response, fibroblast. https://doi.org/10.55463/issn.1674-2974.51.3.12

Background: Alfalfa extract has been shown to have anti-inflammatory activity in wound healing. Objectives: This study aims to investigate the effect of Alfafa on the number of macrophages, fibroblast, and collagen in the rat wound healing. Method: In this post-testonly control group design study, 50 Wistar rats were randomly divided into five groups. K0 (negative control) group was treated with ointment base. K1 (positive control) group was treated with gentamicin. P1, P2, and P3 groups were treated with 10%, 20%, and 30% alfalfa extract respectively. The termination of rats conducted on day 3 and day 7 post of first treatment (5 rats/group/period). On day 3, the number of macrophage and fibroblast were evaluated using Hematoxylin-Eosin staining. On day 7, the number of collagen was evaluated using Siriusred staining. The data were analyzed using one way ANOVA followed by Tukey’s post hoc test. Result: The result, showed a significant difference in the number of macrophages between the negative control treated group and K1 groups. The number of fibroblasts was significantly different between the P3 group and the P1, K1 group. There was a significant difference in the number of collagens between negative control andtreated group, and between the positive control and P2, P3 group. Conclusion: The administration of alfalfa extract at the dose of 20% can accelerate wound healing by increasing the number of fibroblasts and collagen fibers and decreasing number of macrophages. Bangladesh Journal of Medical Science Vol. 22 No. 01 January’23 Page : 77-83

Traumatic ulcer is an open wound in the oral cavity caused by trauma. Flavonoids in ciplukan leaf have an antiinflammatory effect. Drug delivery using mucoadhesive plasters can increase the effectiveness, absorption, and bioavailability of drugs because directly attached to the wound. Lymphocytes can increase macrophage activation that can accelerate wound healing. The purpose of this research is to determine the effect of mucoadhesive plaster of ciplukan leaf extract (Physalis angulata L.) on the number of lymphocytes in healing traumatic ulcers of white rats (Rattus norvegicus). 30 rats were divided into 5 groups; the negative control group, the positive control group and the treatment group with mucoadhesive plaster ciplukan leaf extract doses of 100, 200, 400 mg/kg BW. Conducting heat induction on the labial mucosa of white rats using an amalgam stopper and then given treatment according to groups. The rats were dissected on day 5. The number of lymphocytes was counted on the preparate with Hematoxylin Eosin staining using an Olympus digital microscope with 400x magnification. The treatment group with mucoadhesive plaster of ciplukan leaf extract dose 100 mg/kg BB had the highest number of lymphocytes, while the positive control group had the lowest number of lymphocytes. The conclusion of this research is mucoadhesive plaster of ciplukan leaf extract (Physalis angulata L.) affects the number of lymphocytes in healing traumatic ulcer of white rats (Rattus norvegicus).

Platelet Rich Plasma (PRP) can be used as a regenerative treatment to enhance the activity of growth factors in the blood with the aim of wound healing. PRP can enhance neovascularization, fibroblast formation, and tissue epithelialization more quickly and efficiently. This study aims to determine the histopathological observation of incision wound healing on the skin of white rats given PRP gel. This study used male white rats of the Wistar strain, aged 2-2.5 months and weighing 200-300 grams. The 27 rats used were divided into three treatment groups: P0 (negative control, given 0.9% NaCl solution), P1 (positive control, given Bioplacenton), and P2 (given PRP Gel). The treatment was administered once after the skin had been incised and was given only once. On days 1, 5, and 11, a biopsy of the skin organ was performed for histopathological examination. Histopathological examination includes four indicators: inflammatory cell infiltration, angiogenesis, fibroblasts, and collagen density. Data were analyzed using the Kruskal-Wallis test followed by the Mann-Whitney test, and then described descriptively. The research results show that the infiltration of inflammatory cells and collagen density indicate a difference (P≤0,05) in the group of receiving PRP gel compared to the negative and positive control groups. However, there was no difference in angiogenesis and fibroblasts (P&gt;0.05). In the wound healing process, the histopathological picture of incisional wound healing in the skin of white rats (Rattus norvegicus) given pig blood PRP gel shows an increase and development. Therefore, further research can be conducted to create a more optimal PRP gel formulation, and histopathological examinations can be carried out over a longer observation period to obtain significant results.

Effects of Periploca forrestii Schltr on wound healing by Src meditated Mek/Erk and PI3K/Akt signals

Background and Aim:The capa plant (Blumea balsamifera L.) has been widely used as a traditional herbal medicine in many parts of the world, including South Aceh, Indonesia. It is generally used for wound healing due to its antibacterial and anti-inflammatory properties. However, it is only available as extract or oil, and no gel formulation exists so far. Thus, in this study, we formulated the extract into a pharmaceutical gel and investigated its effectiveness in healing incision wounds in white rats (Rattus norvegicus).Materials and Methods:We collected B. balsamifera leaf samples from Gunongpulo village, South Aceh, Indonesia. We then produced leaf extract through maceration and formulated the extract into a gel using Carbopol 940, methylparaben, triethanolamine, and propylene glycol. We applied the gel to incision wounds in white rats for 7 and 14 days. We then monitored wound healing based on wound length, histology of skin tissues, and levels of cytokine 2 (interleukin-2 [IL-2]).Results:The gel formulation K3 (10% B. balsamifera leaf extract) was the most effective, followed by the gel formulations K2 (5% B. balsamifera leaf extract) and K4 (1% gentamicin ointment, positive control). K3 reduced wound length by 14 mm on day 7 and 29 mm on day 14. Histological analysis showed that fibroblast growth and angiogenesis were most significant in the K3-treated group, exceeding that of the positive control group. The K3-treated group also had the highest IL-2 levels, with an average of 107.7767 ng/L on day 7 and 119.1900 ng/L on day 14.Conclusion:The 10% B. balsamifera leaf gel effectively reduced wound length, increased fibroblast cell growth and angiogenesis, and IL-2 levels, accelerating wound healing.

Indonesia has the largest biodiversity in the world (Megabiodiversity), including Paitan Grass (Paspalum conjugatum) or what will hereinafter be called P. conjugatum, which is a forage plant for livestock (HPT). In several regions such as the Philippines, Kalimantan, Papua New Guinea and Manggarai, this grass is commonly used as a wound medicine. This study aims to determine the effectiveness of Paitan Grass leaf extract on incisional wounds in white rats. The method used was laboratory experimental, using Paitan Grass (P. conjugatum) which was extracted using a maceration technique with 70% ethanol solvent for 3 days, and applied to white rats (Rattus norvegicus) which had been incised in vivo. This study had 3 groups, namely positive control (povidone iodine 10%), negative control (NaCl 0.9%), and Paitan Grass extract. The parameters for observing incisional wound healing in this research were the length of time, speed of hemostasis, wound color, exudate and crusting, and the length of time for wound healing which was measured for 14 days. Measured using a caliper. The results obtained were analyzed using One Way ANNOVA Tukey HSD advanced test. The results showed that Paitan Grass (P. conjugatum) extract was effective in speed of hemostasis (P&gt;0.05) with positive control, color change in 48 hours, exudate visible in 24 hours, crusting and speed of wound healing (P&lt;0.05 ) with both control groups, but there was no significance (P&gt;0.05) between the positive control and negative control.