Abstract
Bioprotein production is one of the most promising breakthroughs of biotechnological innovations. Due to its increasing demand, the efficient strains, substrate and method must be used for high yield product. In this study, screening of five different cerivisiea, Mucor hiemalis and Thricoderma harzianum, was done for bioprotein production by liquid state bioconversion of wheat flour as a cheaper carbon source. Bioconversion was done with fixed wheat flour concentration of 2% (w/v) at a temperature 27°C, agitation of 150 rpm with 2% inoculum (106 spores mL-1). Biomass production was recorded continuously for six days and the protein content was also determined every day. From the observed results, Mucor hiemalis was found to be the most potential strain with biomass of about 11.48 g L-1 on the fourth day of treatment. With this promising result, the amount of bioprotein was further increased to 21.89 g L-1 by optimizing few process factors. Further optimization developments are in progress. This study may provide a better alternative in agricultural products by converting cheaper carbon source to valuable and quality product bioprotein, which can be used as supplement and additive in the animal feed and food as well as in chemical and pharmaceutical industries.
Highlights
The significant increase in demand for livestock products in recent years in developing countries has required an increase in animal and human food supply
T. harzianum and M. hiemalis obtained the optimum biomass of 10.7 g L1 and11.4 g L1 respectively on 4th
Media optimization for bioprotein production: After selecting M. hiemalis as the potential strain for the bioprotein production using wheat flour as a substrate, we evaluated its performance by improving media compositions
Summary
The significant increase in demand for livestock products in recent years in developing countries has required an increase in animal and human food supply. The importance of protein as food nutrient cannot be ignored because its deficiency can cause various malnutrition problems This demands a search for new protein sources, with high nutritional value, economically feasible and locally available. The production of bioprotein (protein derived from micro-organisms) by fermentation of wheat flour-a cheaper carbon source, is one of the most promising breakthrough of biotechnological innovations. This will certainly increase the availability of affordable good quality protein in the world. Various malnutrition problems may occur due to protein shortage This situation has created a demand for the formulation of innovative and alternative protein-rich food sources[2]. The food route represents highest immediate cash return because demand for food is huge and will remain stable and the technologies involved are too cost effective
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