Abstract
Elymus sibiricus as an important forage grass and gene pool for improving cereal crops, that is widely distributed in West and North China. Information on its genetic diversity and relationships is limited but necessary for germplasm collection, conservation and future breeding. Start Codon Targeted (SCoT) markers were used for studying the genetic diversity and relationships among 53 E. sibiricus accessions from its primary distribution area in China. A total of 173 bands were generated from 16 SCoT primers, 159 bands of which were polymorphic with the percentage of polymorphic bands (PPB) of 91.91%. Based upon population structure analysis five groups were formed. The cluster analysis separated the accessions into two major clusters and three sub-clusters, similar to results of principal coordinate analysis (PCoA). The molecular variance analysis (AMOVA) showed that genetic variation was greater within geographical regions (50.99%) than between them (49.01%). Furthermore, the study also suggested that collecting and evaluating E. sibiricus germplasm for major geographic regions and special environments broadens the available genetic base and illustrates the range of variation. The results of the present study showed that SCoT markers were efficient in assessing the genetic diversity among E. sibiricus accessions.
Highlights
IntroductionElymus L. is the largest genus of the tribe Triticeae and includes about 150 species worldwide [1]
Elymus L. is the largest genus of the tribe Triticeae and includes about 150 species worldwide [1].Elymus sibiricus (Siberian wild rye) is one of the most important species of the genus
The present study examines the level of genetic diversity and its molecular variation of 53 E. sibiricus accessions from five geographic regions in China
Summary
Elymus L. is the largest genus of the tribe Triticeae and includes about 150 species worldwide [1]. Further analysis of the genetic diversity and variation of Chinese wild E. sibiricus accessions, cultivars and breeding lines from species range and their comparison may produce new insights and give a better understanding of the distribution of genetic diversity. The purposes of this study were: (a) to assess the genetic diversity and phylogenetic relationship among 53 E. sibiricus accessions; and (b) to examine the effectiveness of the SCoT markers in E. sibiricus genetic diversity study.
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