Abstract
BackgroundBovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle.ResultsPresent findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP®) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. The specificity of the assay ranged from 98.9% (n = 92, US) to 96.0% (n = 50, GB) with samples from TB-free herds. Multi-antigen print immunoassay (MAPIA) revealed the presence of antibodies to multiple antigens of M. bovis in sera from TST non-reactors diagnosed with TB.ConclusionsThus, use of serologic assays in series with TST can identify a significant number of TST non-reactive tuberculous cattle for more efficient removal from TB-affected herds.
Highlights
Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle
Antibody detection in TST non-reactors versus TST reactors: Samples from Great Britain With sera from M. bovis infected cattle in GB, the overall IgG reactivity rates in the Dual path platform (DPP) assay were similar for single intradermal comparative cervical test (SICCT) reactors (60%, n = 75) and non-reactors (65.4%, n = 52)
The immunodominance of serologically related Mobility protein of bovis (MPB70) and/or Mobility protein of bovis (MPB83) proteins typically detected in M. bovis infected cattle after purified protein derivative (PPD) administration for SICCT was less evident with TST non-reactors, as reactivity rates to Culture filtrate protein 10 kDa protein (CFP10)/Early secretory antigenic target 6 kDa protein (ESAT-6) and MPB70/MPB83 were essentially equivalent, and there were considerably more CFP10/ESAT-6 antibody responders within the TST non-reactor subset
Summary
Bovine tuberculosis (TB) control programs generally rely on the tuberculin skin test (TST) for ante-mortem detection of Mycobacterium bovis-infected cattle. Results: Present findings demonstrate that a rapid antibody test based on Dual-Path Platform (DPP®) technology, when applied 1-3 weeks after TST, detected 9 of 11 and 34 of 52 TST non-reactive yet M. bovis-infected cattle from the US and GB, respectively. Despite intensive and costly control efforts over many decades, bovine TB persists in most countries adversely affecting animal health, welfare, and trade as well as the livelihoods of producers. Control strategies rely largely on ante-mortem testing and slaughter inspection to identify livestock herds at risk. The principal ante-mortem tests for Waters et al BMC Veterinary Research (2017) 13:164 to atypical mycobacteria, and many other factors [4, 5]. Improved ante-mortem tests and/or testing strategies for bovine TB are greatly needed
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