Potential for biodegradation of anthropogenic organic compounds at low temperature in boreal soils

Abstract

The effect of temperatures of −2.5 to +20 °C on the biodegradation of concentrations 0.2–50 μg cm −3 of pentachlorophenol (PCP), phenanthrene, pyrene and 2,4,5-trichlorophenol (TCP) was studied in soils sampled from an agricultural field and a relatively pristine forest in Helsinki, Finland. At the temperatures simulating seasonal variation of boreal soil temperatures [Heikinheimo, M., Fougstedt, B., 1992. Statistic of Soil Temperature in Finland. Meteorological Publications 22. Finnish Meteorological Institute, Helsinki, Finland], the response of mineralization of PCP, phenanthrene and 2,4,5-TCP was the most effective in the rhizosphere fraction of the forest humus soil at the substrate concentrations of ⩽5 μg cm −3. In the control incubation, performed at constant temperature of +20 °C, the mineralization yields of the model pollutants were highest in the agricultural soil with the highest applied substrate concentration (50 μg cm −3). The results suggest that the high level of pollutant mineralization at +20 °C resulted from the apparent adaptation of the soil microbial community to the high substrate concentration. No such adaptation occurred when the soils were incubated at temperatures simulating the actual boreal soil temperatures. The present results stress the role of adjusting the incubation conditions to environmentally relevant values, when assessing biodegradation of anthropogenic organic compound in boreal soils.