Potential for artificial symbiosis between marine microalgae and invertebrates: II. survival of marine mollusks with the transplanted dinof lagellate <i>Effrenium voratum</i> (Symbiodiniaceae)

Key features: Serves as a standard reference for researchers, teachers and students of various disciplines such as Fisheries Science, Marine Biology, Life Sciences, Biotechnology, Natural Products, Biochemistry, and Pharmacy, their libraries, and as a valuable guide for pharmaceutical companies involved in the development of new drugs Presents the taxonomy, common name, global distribution, and habitat of 275 species of marine molluscs Provides the diagnostic features and pharmaceutical compounds (along with their chemical structure and activities) of 275 species of marine molluscs Includes more than 500 detailed illustrations Covers bivalves, gastropods, and cephalopods Biology and Ecology of Pharmaceutical Marine Molluscs is the first comprehensive book of its kind written by scientists from both the Marine Biology and Pharmacy disciplines to fill the long-felt need for a marine natural products book devoted exclusively to molluscs. The taxonomy, common name, global distribution, habitat, diagnostic features and pharmaceutical compounds (along with their activities) of 275 species of marine molluscs are given in this comprehensive work, with accompanying illustrations. Bioactive substances isolated from marine invertebrates such as poriferans, coelenterates, molluscs, echinoderms, bryozoans and tunicates have been reported to possess antiviral, antimicrobial, antiprotozoal, antifungal, antihelmintic and anticancer activities. Among the marine invertebrates, marine molluscs have significant commercial applications; for instance, as a source of food, ornaments and production of lime. A few species of molluscs have also been reported to act as scavengers in cleaning the environment and pollution indicators. Recently, many molluscan species have been identified as a potential source of secondary metabolites, having a wide range of pharmaceutical applications. This should be a standard reference for students, researchers and teachers of disciplines such as Pharmacy, Fisheries Science, Marine Biology, Life Sciences, Biotechnology and Biochemistry, as well as a valuable guide for pharmaceutical companies involved in the development of new drugs from marine molluscs.

Sunderban Biosphere Reserve (SBR) is the largest existing mangrove ecosystem in the world harbouring 177 species of Mollusca. During several surveys in Sunderban Biosphere Reserve to record the faunal diversity, five molluscan species, namely Dosinia tumida (Gray, 1838), Babylonia spirata (Linnaeus, 1758), Babylonia zeylanica (Bruguière, 1789), Indothais gradata (Jonas, 1846), Architectonica perdix (Hinds, 1844) have been identified as new records from the region. Also, D. tumida and B. zeylanica have been identified as new for the faunal diversity of West Bengal. Another one, I. gradata is being recorded from India by this communication for the first time. The recorded molluscan diversity from Indian Sunderban and these present findings imply that malacofaunal study from SBR need to be reassessed for an updated malacofaunal diversity status for implementing better conservation tactics in SBR.

PurposeThe purpose of this paper is to evaluate the proximate composition, amino acid and mineral profiles of seafood for human consumption.Design/methodology/approachA total of 21 seafood species (eight seawater, one fresh water fish, six crustacean and six mollusc species) of commercial importance were chosen and purchased from the Istanbul local fish market. The sample to amino acids analyze was prepared in accordance with the hydrolysis technique described by Waters AccQ.Tag Chemistry Package Method (HPLC). Determination of iron (Fe), sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), selenium (Se), phosphorus (P) and iodine (I) was performed with thermo electron X7 inductively coupled plasma mass spectrometry (ICP‐MS).FindingsThe lipid contents of species were found to be very low and considered as lean. The highest total amino acid values of fishes, crustaceans and molluscs were determined in John Dory, hake, red scorpion fish, spiny lobster, Norway lobster, sea snail and pecten. The mineral content of seafood species were found to be 9.3‐157.11 mg/kg Fe, 558.13‐6095.89 mg/kg Na, 253.25‐1032.29 mg/kg Mg, 125.43‐17174.76 mg/kg Ca, 0.18‐7.76 mg/kg Se, 1586.45‐5811.16 mg/kg P and 0.086‐2.630 mg/kg I.Originality/valueThis paper is helpful to consumers and academics concerning the proximate, amino acid and mineral composition of 21 estimable seafood species (nine fish, six crustacean and six mollusc species).

Gut bacteria of phytophagous and omnivorous marine invertebrates often possess alginate lyases (ALGs), which are key enzymes for utilizing macroalgae as carbon neutral biomass. We hypothesized that the exclusive feeding of a target alga to marine invertebrates would shift the gut bacterial diversity suitable for degrading the algal components. To test this hypothesis, we reared sea hare (Dolabella auricularia) and sea snail (Batillus cornutus) for two to four weeks with exclusive feeding of a brown alga (Ecklonia cava). Pyrosequencing analysis of the gut bacterial 16S rRNA genes revealed shifts in the gut microbiota after rearing, mainly due to a decrease in the variety of bacterial members. Significant increases in six and four 16S rRNA gene phylotypes were observed in the reared sea hares and sea snails, respectively, and some of them were phylogenetically close to known alginate-degrading bacteria. Clone library analysis of PL7 family ALG genes using newly designed degenerate primer sets detected a total of 50 ALG gene phylotypes based on 90% amino acid identity. The number of ALG gene phylotypes increased in the reared sea hare but decreased in reared sea snail samples, and no phylotype was shared between them. Out of the 50 phylotypes, 15 were detected only after the feeding procedure. Thus, controlled feeding strategy may be valid and useful for the efficient screening of genes suitable for target alga fermentation.

10 - Biochemical Interactions between Molluscs and Their Algal and Bacterial Symbionts

One way to identify an object is to make observations to a place to find objects that will be examined, in this article we take invertebrate animal objects in the mollusca phylum on Kenjeran Beach Surabaya. Phylum mollusca are a group of soft-bodied and invertebrate animals with shell-protected bodies. Data collection was carried out using exploration and observation methods, with research data analysis techniques using descriptive analysis techniques. 5 species of mollusca were obtained on the Kenjeran Coast, namely sea snails (Bolinus brandaris), Hiatella arctica, Sorgenfreispira brachystoma, Eupleura caudata and Keoang mas (Pomacea canaliculate). The existence of this study aims to recognize invertebrate animals in the mollusk phylum and the important characteristics of the species we found there.

On the evenings of June 11 and 12, 2019, 5 and 6 days before full moon, broadcast spawning by four echinoderm species and two mollusc species was observed on the Marsa Shagra reef, Egypt (25° 14′ 44.2" N, 34° 47′ 49.0" E). Water temperature was 28 °C and the invertebrates were observed at 2–8 m depth. The sightings included a single basket star Astroboa nuda (Lyman 1874), 2 large Tectus dentatus (Forskal 1775) sea snails, 14 individuals of the Leiaster cf. leachi (Gray 1840) sea star and 1 Mithrodia clavigera (Lamarck 1816) sea star, 3 Pearsonothuria graeffei (Semper 1868) sea cucumbers, and 2 giant clams, Tridacna maxima (Röding 1798). The observations presented here provide relevant information on broadcast spawning of non-coral invertebrate taxa in the Red Sea, where spawning is considerably less well documented than in other tropical geographical regions such as the Indo-Pacific and Caribbean.

Shellfish processing wastewater: characterization of a group of wastewater resources for future valorisation.

Microbes associated with marine invertebrates play a key role in the physiological and biochemical processes of the host, and can be responsible for food-borne diseases in humans. Raw invertebrates are a common component of coastal gastronomy worldwide and their consumption could represent a potential risk to humans if their microbiome hosts infectious bacteria. However, these species’ microbiome composition is usually unknown. In this study, we sequenced the 16S gene to characterise the microbiome of the digestive system and gonads of the commercially-exploited sea snail Phorcus sauciatus from the Macaronesian islands and mainland Portugal. The goal was to identify bacteria that might pose a threat to humans. In total, 910 OTUs were identified, thirty-two of which were found to be classified as Risk level-1 and -2 species. Among these, twenty pathogenic bacterial strains were found in high relative abundance and identified as potential drivers of human diseases, including Micrococcus luteus and Serratia marcescens. Here, we discuss how our findings on the occurrence of these bacteria could seriously affect humans. Our results are relevant beyond the scope of this study, as this work might also pave the way for uncovering further implications on the raw consumption of other shellfish and invertebrate species.

Regulatory mechanism of marine endosymbiosis in Cnidarian-dinoflagellate association remains unknown. In order to investigate cellular and molecular interactions between symbionts and their host endoderm cells, an homogeneous population of endoderm cell is required. We explore a novel strategy to isolate endoderm cells, based on the polar distribution of different tissues in tentacles of stony coral Euphyllia glabrescens. Inversion of tentacle fragments re-arranges the orientation of endoderm. Change in orientation results in dissociation of both symbiotic and aposymbiotic endoderm cells upon mechanical swirling within an hour. The homogeneity of collected endoderm cells was found to be higher than 95%. Microscopic examination of dissociated symbiotic endoderm cells indicated the presence of multiple numbers of symbionts within a single endoderm cell. Membrane labeling using fluorescent probe FM1-43 demonstrates membrane trafficking between the endoderm cell membrane and the symbiosome membrane that encloses the symbiont. In addition, the fluorescent study revealed significant membrane internalization in aposymbiotic endoderm cells, suggesting a dynamic nature of membrane in these cells. The inverted manipulation of the tentacles also provides an in vitro system to in estigate effects of host tissue arrangement on the photosynthetic activity of resident symbionts. The present work not only provides a new cell preparation for the study of molecular and cell regulation of endosymbiosis in marine invertebrates, but also sheds new insight on the relationship of tissue arrangement and symbiotic activity.

The coevolution of hosts and symbionts based on virulence and mode of transmission is a complex and diverse biological phenomenon. We introduced a conceptual model to study the stable coexistence and coevolution of an obligate symbiont (mutualist or parasite) with mixed-mode transmission and its host. Using an age-structured Leslie model for the host, we demonstrated how the obligate symbiont could modify the host’s life history traits (survival and fecundity) and the long-term growth rate of the infected lineage. When the symbiont is vertically transmitted, we found that the host and its symbiont could maximize the infected lineage’s evolutionary success (multi-level selection). Our model showed that symbionts’ effect on host longevity and reproduction might differ, even be opposing, and their net effect might often be counterintuitive. The evolutionary stability of the ecologically stable coexistence was analyzed in the framework of coevolutionary dynamics. Moreover, we found conditions for the ecological and evolutionary stability of the resident host-symbiont pair, which does not allow invasion by rare mutants (each mutant dies out by ecological selection). We concluded that, within the context of our simplified model conditions, a host-symbiont system with mixed-mode transmission is evolutionarily stable unconditionally only if the host can maximize the Malthusian parameters of the infected and non-infected lineages using the same strategy. Finally, we performed a game-theoretical analysis of our selection situation and compared two stability definitions.

Marine invertebrates are a paraphyletic group that comprises more than 90% of all marine animal species. Lipids form the structural basis of cell membranes, are utilized as an energy reserve by all marine invertebrates, and are, therefore, considered important indicators of their ecology and biochemistry. The nutritional value of commercial invertebrates directly depends on their lipid composition. The lipid classes and fatty acids of marine invertebrates have been studied in detail, but data on their lipidomes (the profiles of all lipid molecules) remain very limited. To date, lipidomes or their parts are known only for a few species of mollusks, coral polyps, ascidians, jellyfish, sea anemones, sponges, sea stars, sea urchins, sea cucumbers, crabs, copepods, shrimp, and squid. This paper reviews various features of the lipid molecular species of these animals. The results of the application of the lipidomic approach in ecology, embryology, physiology, lipid biosynthesis, and in studies on the nutritional value of marine invertebrates are also discussed. The possible applications of lipidomics in the study of marine invertebrates are considered.

Abstract

Mangrove is an evergreen, salt tolerant plant community, which grows in inter-tidal coastal zones of tropical and subtropical regions of the world. They are ecologically important for many fauna species and are rich in food resources and consist of many different vegetation structures. They serve as ideal foraging and nursery grounds for a wide array of species such as birds, mammals, reptiles, fishes and aquatic invertebrates. In spite of their crucial role, around 50% of mangrove habitats have been lost and degraded in the past two decades. The fauna diversity of mangrove habitat at Marudu Bay, Sabah, East Malaysia was examined using various methods: i.e. aquatic invertebrates by swap nets, fish by angling rods and cast nets, reptiles, birds, and mammals through direct sighting. The result showed that Marudu Bay mangrove habitats harbored a diversity of fauna species including 22 aquatic invertebrate species (encompassing 11 crustacean species, six mollusk species and four worm species), 36 fish species, 74 bird species, four reptile species, and four mammal species. The wide array of fauna species could be due to the availability of complex vegetation structures, sheltered beaches and tidal mudflats, which are rich in food resources and also offer safe foraging and breeding grounds for them. These heterogeneous habitats must be protected in a sustainable way in order to ensure the continued presence of aquatic and terrestrial fauna species for future generations.

This comprehensive volume focuses exclusively on sea snails (or gastropods), which are popular food items and occupy an important role in the commercial shell craft industry. Familiar examples include conchs (highly sought after due to their mild flavor) and escargot, abalone, and periwinkle snails. This book covers the profile (habitat, distribution, morphology, food and feeding, reproduction, conservation status, etc.) of about 180 species of commercial edible marine gastropod molluscs as well as their nutritional values, commercial importance, and pharmaceutical value. Also included is information on their prevalent diseases and parasites. The informative descriptions are presented in an easy-to-read style with neat illustrations.