Potential aversive compounds in leafy spurge for ruminants and rats

Abstract

Several wild and domestic ruminant species and horses apparently will not consume leafy spurge (Euphorbia esula) while grazing range and pasture lands. It has been demonstrated that leafy spurge can elicit conditioned food aversions in cattle and sheep, and the aversion-eliciting capacity of leafy spurge may account for why cattle seldom graze this nutritious plant and why sheep may not readily consume it at some locations. The identity of the aversive compound(s) in leafy spurge is unknown, but several different diterpenoid ingenol esters have been isolated from its tissues, and we suspect that one or more ingenol esters may be aversion-eliciting compounds in leafy spurge. The objectives of this study were to determine whether or not leafy spurge is aversive to laboratory rats and if a crude acetone extract of leafy spurge, presumably containing ingenol esters and other phytochemicals, could generate an aversive response in sheep and laboratory rats. An additional objective was to determine whether or not a particular ingenol monobenzoate, which may be similar to ingenol esters in leafy spurge, might also elicit an aversive response from rats. Rats exhibited food aversions associated with leafy spurge (P < 0.05). An acetone extract of leafy spurge induced conditioned food aversions in both sheep and rats (P < 0.01). The ingenol 3-monobenzoate also induced conditioned food aversions in rats (P < 0.01). Our interpretation of these data is that rats can be used as a model for cattle and sheep with respect to their aversion to leafy spurge ingestion. Additionally, we suggest that one or more ingenol esters may be aversion-inducing agents in leafy spurge. However, others may exist in leafy spurge that are also aversive or are the only or prime aversive chemicals.