Abstract
Vaccine candidate tests were carried out in the Laboratory of Microbiology, Faculty of Agriculture ULM; and the Laboratory of Molecular Genetics, Faculty of Science and Technology Airlangga University. The field test was conducted in Tampakang Village, Hulu Sungai Utara district and Bati-Bati village, Tanah Laut district that was including sterility test through vaccine mediated <em>Brain Heart Infusion </em>(BHI) inoculation. Safety testing was applied in experimental animals (mice and swamp buffaloes). Serum was tested serologically using <em>Enzyme Linked Immuno Sorbent Assay</em> (ELISA). Vaccine safety test showed 100% of mice and buffalo are surviving without clinical symptoms according to the characteristics of hemorrhagic septicemia disease. ELISA test indicated vaccinations increase antibody production, namely the <em>Optical Density</em> (OD) ƛ 450 ƞm 0.292 before vaccination to be 0.748 and 1.576 after vaccination and to be 1.821 after boosted. Based on sterilization test, safety test, and vaccine potency test, the vaccine candidate from cell wall proteins of <em>P. multocida</em> local isolate can be used to prevent hemorrhagic septicemia disease that infect swamp buffalo both on laboratory and field experiment.
Highlights
Constraints faced by buffaloes in South Kalimantan vary, such as reduced grazing land which results in a reduced availability of natural food which causes buffalo body defenses to be reduced so that it is susceptible to disease, one of these diseases is Pasteurellosis caused by the bacterium Pasteurella multocida (P. multocida) and its variants
It can be concluded that sample bacteria was stored in the microbial laboratory of the Agriculture Faculty ULM and tested its pathogenesis on Balb C mice was Pasteurella multocida bacteria, this finding confirms to statement of De Alwis (1992) end Woolcock (1992), that Pasteurella multocida is bacteria with shorth stick form, size 1 – 2 μm X 0.3 – 1.0 μm, and gram-negative
The bacteria looks red Bacteria are small rods and both ends are rounded Under a microscope, bacteria appear to be entirely blue The bacteria form small, round, and serrated colonies In blood agar media, faded red does not occur Bacteria only grow on puncture marks Change media color from green to yellow Change media color from red to yellow Change media color from red to yellow Change media color from red to yellow Red ring is formed Not change media color the color on the oxidation stick turns blue Seen bubbles on the media after adding H2O2 solution
Summary
Constraints faced by buffaloes in South Kalimantan vary, such as reduced grazing land which results in a reduced availability of natural food which causes buffalo body defenses to be reduced so that it is susceptible to disease, one of these diseases is Pasteurellosis caused by the bacterium Pasteurella multocida (P. multocida) and its variants. This bacterium can cause serious illness; this organism can infect many wild and domestic animal species, and can cause acute or chronic diseases (Alwis, 1992) in Herliani and Sulaiman (2011).
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