Abstract
Organic and aqueous extracts of the Trichilia pallida Swartz (Meliaceae) were obtained for identification of fractions with insecticidal activity against the tomato leafminer Tuta absoluta (Meyrick). Leaf and twig freeze-dried aqueous extracts (FDA) were resuspended in water at 3% and sprayed, with the aid of a mini-atomizer, over tomato leaflets infested with newly-hatched larvae. Larval mortality at 5 and 10 days after infestation (DAI) were higher for leaf extracts. In a second set of experiments, leaf extracts at 1% (w/v) were obtained by maceration in hexane (HEX), dichloromethane (DIC) and methanol (MET), and tested as described above using acetone and water as control. DIC extracts were the most promising as a source of substances with insecticidal activity against larvae of T. absoluta. DIC leaf extracts were further processed and subjected to partition assays, producing fractions in HEX, MET, ethyl acetate (ETA), n-butanol (NBU) and water (WA). The 0.15% ETA and MET fractions obtained from the partition assay of DIC extracts showed the highest insecticidal activity against T. absoluta.
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