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Abstract
Abstract Bovine semen cryopreservation is a fundamental procedure in animal reproduction. However, it is associated with the production of reactive oxygen species (ROS), which can cause significant damage to spermatozoa, leading to infertility and reduced sperm quality. In this context, using seminal diluents enriched with antioxidants presents a promising strategy for minimizing or even reversing these harmful effects. This study aimed to evaluate the effects of different concentrations of pequi (Caryocar coriaceum) esters as a supplement to bovine semen cryopreservation diluents. Forty-two ejaculates were collected from six bulls that had undergone an andrological examination and were deemed suitable. The samples were analyzed, diluted in Tris-egg yolk medium, supplemented with pequi esters (0.0, 0.5, 1.0, and 1.5 mL/L), packaged, and cryopreserved. The sperm parameters evaluated in the post-thaw semen included the thermoresistance, plasma membrane functionality, cleavage and blastocyst rates, and quantification of reduced glutathione and malondialdehyde. The results showed that the concentrations of 1.0 and 1.5 mL/L of pequi esters significantly reduced malondialdehyde levels in cryopreserved semen. However, no significant effects of pequi esters were observed on motility parameters, sperm functionality, cleavage and blastocyst rates, or on the quantification of reduced glutathione. Supplementation with pequi ester (1.0 and 1.5 mL/L) in the cryopreservation of bovine semen may be beneficial in minimizing lipid peroxidation effects, as evidenced by the reduction in malondialdehyde concentration after thawing.
Published Version
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