Abstract
ObjectiveThe aim of this study was to evaluate the folliculogenesis progression in mice ovaries after vitrification/devitrification followed by ectopic autotransplantation.MethodsFemale Balbc mice (60days, n=12), were anesthetized and bilaterally ovariectomized. One ovary was kept in buffer (PBS) during the procedure (control group – fresh). The other ovary was placed in PBS and then vitrified/devitrified using the IngáMed® kits, following the manufacturer’s instructions. Both ovaries were subcutaneously transplanted and after 21 days some animals (n=6) received 5UI of eCG (equine chorionic gonadotropin, Novormon 5000®) and, after 48h, 5UI of hCG (human chorionic gonadotropin, Vetecor®), analogous to FSH and LH respectively; other animals received PBS (n=6; control). Three days later, the hosts were euthanized and grafts were recovered, processed to histology (HE) and qualitatively analyzed.ResultsPrimordial, primary, multilaminar and antral follicles were observed in fresh ovaries, with or without hormonal stimulation. In vitrified/devitrified tissues without stimulation, folliculogenesis were observed up to the multilaminar stage and in those with exogenous stimulation, a higher follicular density was observed, with follicles in preovulatory stage. Atretic follicles also were observed in all groups.ConclusionsFolliculogenesis progression was observed in fresh and vitrified tissues; however, hormonal superstimulation using exogenous gonadotropins brought better results. This study showed, for the first time in the literature, the feasibility of the ovarian tissue vitrification technique, without morpho- functional damages. So, this technique could be used for fertility preservation and should be more investigated.
Highlights
In vitro maturation (IVM) is an option for infertile couples, especially for women with polycystic ovary syndrome (PCOS)
The results demonstrate the importance of re-biopsy and preimplantational genetics screening (PGS) in previously biopsied embryos chromosomally analyzed by analysis by Comparative Genomic Hybridization (aCGH), which had DNA amplification failure in first analysis
We have sucessfully developed a Facebook page used by reproductive age men and women to obtain information related to endometriosis and infertility
Summary
In vitro maturation (IVM) is an option for infertile couples, especially for women with polycystic ovary syndrome (PCOS). Comments: IVM is a promising technique when applied to PCOS patients providing good quality blastocysts which, after proper vitrification, may be warmed and transferred resulting in a healthy pregnancy. 30 years old, BMI=26.1, has no history of male factor They passed through five IVF previous cycles elsewhere, four did not result in pregnancy and the fifth had no transfer because all biopsied embryos were aneuploid. Case Report: A 37-year-old female, indicated for in vitro fertilization (IVF) due to bilateral tubal factor, initiated ovulation induction on day 2 of the menstrual cycle. One day later, she traveled with her mother and sister, taking the medications. There would have to be a maternal meiosis error with failure to extrude the second polar body
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