Objectives: Anatomic anterior cruciate ligament (ACL) reconstruction is defined as the restoration of the native insertion sites, tissue dimensions, and collagen orientation. However, the microstructure and composition of standard tendon grafts used for ACL reconstruction differs from ligament. A bone-ACL-bone allograft (B-ACL-B) could represent a potential solution to ACL reconstruction, and thus the purpose of this study is to evaluate incorporation of a B-ACL-B graft used for ACL reconstruction in a rabbit model. Methods: Our study was approved by the Institutional Animal Care and Use Committee. A total of 71 New Zealand white rabbits were used, with 30 donor rabbits for harvesting bilateral B-ACL-B allografts, and recipient rabbits undergoing unilateral ACL resection followed by reconstruction with B-ACL-B allograft. Animals were euthanized for biomechanical testing (n = 14) at 4 and 8 weeks after surgery, and for micro–computed tomography (μCT), multi-photon microscopy, transmission electron microscopy (n=12), and histological analysis (n= 15) at 2, 4 and 8 weeks after surgery. Multi-photon microscopy analysis used Fast Fourier Transform in imageJ to calculate the proportion of fibers that deviated from orthogonal as a measure of collagen alignment and organization. One-way analysis of variance (ANOVA) with the post-hoc Tukey test was used to analyze differences among groups. Results: Gross inspection and radiographs confirmed intact ACL allograft in the proper anatomic position without evidence of complication. There w...
Anatomic Anterior Cruciate Ligament Reconstruction Anatomic Anterior Cruciate Ligament Bone Tunnel Native Anatomic Anterior Cruciate Ligament Bone Block Bone Tunnel Wall Multi-photon Microscopy Analysis Tunnel Wall Multi-photon Microscopy Institutional Animal Care
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Climate change Research Articles published between Nov 21, 2022 to Nov 27, 2022
Nov 28, 2022
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No potential conflict of interest was reported by the authors. The conception and design of the study, acquisition of data, analysis and interpretatio...Read More
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