Abstract
Addition of Zn 2+_ to cell medium inhibited the induction of ornithine decarboxylase (ODC) activity in ODC overproducing L1210-DFMO r cells. A significant effect was observed at a concentration as low as 0.01 mM, however a more marked inhibition was caused by the addition of 0.1 mM Zn 2+. The inhibition of the induction of ODC activity was accompanied by a proportional decrease in the content of immunoreactive ODC protein, whereas the level of ODC mRNA, detemined by a solution hybridization RNase protection assay, was not affected signigicantly. Instead, some acceleration of ODC turnover was observed. the addition of 0.1 mM Co 2+ or Mn 2+, but not of other divalent metal ions, also inhibited ODC induction; differently from Zn 2+ however, these metals affected cell viability and/or cell growth. Removal of endogenous Zn 2+ by a chelator also provoked a strong decrease of ODC induction, which was reversed by Zn 2+. However, addition of Zn 2+ in excess of the chelator proved to be markedly inhibitory. These results indicate that both a restricted Zn 2+ availability and an enhanced presence of the metal can inhibit the induction of ODC in L1210-DFMO r cells.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
