Possibility for Exploitation and Identification of Rice Yield and Seed Quality Orthologs in Peanut (Arachis hypogaea L.)

Abstract

Background: The advancement in gene tagging and mapping in model crops such as Arabidopsis, rice andmaize has outpaced progress in major legume crops like groundnut, chickpea, redgram, greengram andsoybean, even in the post-genome release period of legume databases. To address this gap, a study investigated the transferability of gene tagged markers (GTMs) associated with rice yield, grain size andmicronutrient content (Fe and Zn) to groundnut. Methods: This study utilized 17 diverse groundnut genotypes for molecular analysis, targeting 45 GTMs spanning 24 known functional genes. Result: Remarkably, 31 out of the 45 markers (76%) demonstrated high transferability to groundnut, indicating significant conservation in functional regions across species. The amplification efficiency of rice GTMs varied among groundnut genotypes, ranging from 79.17% to 91.67%. Notably, 17 markers targeting 14 rice genes showed amplification across all 17 groundnut genotypes (100%) studied. These findings underscore the potential of leveraging knowledge from functionally characterized genes in molecular model crops and annotated orthologous genes in ‘Omics’ databases to enhance gene tagging efforts in crops like groundnut. By harnessing this approach, there is an opportunity to expand the molecular-level tagging of genes and improve crops like groundnut, which have seen limited progress in gene tagging. This strategy opens avenues for pyramiding desirable genes with precision, thereby enhancing crop traits with greater accuracy.