Abstract
Crop diseases cause great harm to food security, 90% of these are caused by fungal spores. This paper proposes a crop diseases spore detection method, based on the lensfree diffraction fingerprint and microfluidic chip. The spore diffraction images are obtained by a designed large field of view lensless diffraction detection platform which contains the spore enrichment microfluidic chip and lensless imaging module. By using the microfluidic chip to enrich and isolate spores in advance, the required particles can be captured in the chip enrichment area, and other impurities can be filtered to reduce the interference of impurities on spore detection. The light source emits partially coherent light and irradiates the target to generate diffraction fingerprints, which can be used to distinguish spores and impurities. According to the theoretical analysis, two parameters, Peak to Center ratio (PCR) and Peak to Valley ratio (PVR), are found to quantify these spores. The correlation coefficient between the detection results of rice blast spores by the constructed device and the results of microscopic artificial identification was up to 0.99, and the average error rate of the proposed device was only 5.91%. The size of the device is only 4 cm × 4 cm × 5 cm, and the cost is less than $150, which is one thousandth of the existing equipment. Therefore, it may be widely used as an early detection method for crop disease caused by spores.
Highlights
Rice is the most important crop all around the world
Rice blast disease, which is caused by a kind of spore named Magnaportheoryzae [3], affects most of the rice-producing countries and has already spread to approximately 85 countries [4]
Microfluidic chip has many advantages, such as little reagent consumption, fast automatic detection, low cost, high integration, and has allowed researchers concentrate the whole process of a biochemical reaction on the chip [17]—mostly applied in the field of liquid and medical testing [18]
Summary
Rice is the most important crop all around the world. Currently, approximately 5.6 billion people (80% of the world’s population) have rice as their staple food. The common method is to visually measure and count the spores under microscope [10], but the microscope is expensive and bulky, and cannot be widely used outdoors Most of these methods require complex procedures and multiple reagents, resulting in low experimental repeatability [11]. Microfluidic chip has many advantages, such as little reagent consumption, fast automatic detection, low cost, high integration, and has allowed researchers concentrate the whole process of a biochemical reaction on the chip [17]—mostly applied in the field of liquid and medical testing [18]. According to the above technology, a spore detection device has been developed, which includes the microfluidic chip and lensless imaging module. It can enrich and detect spores and realize early monitoring of crop diseases
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