Abstract
(1) Background: Access to clean water is a very important factor for human life. However, pathogenic microorganisms in drinking water often cause diseases, and convenient/inexpensive testing methods are urgently needed. (2) Methods: The reagent contains 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and phenazine methosulfate (PMS) and can react with succinate dehydrogenase within bacterial cell membranes to produce visible purple crystals. The colorimetric change of the reagent after reaction can be measured by a sensor (AS7262). (3) Results: Compared with traditional methods, our device is simple to operate and can provide rapid (i.e., 5 min) semi-quantitative results regarding the concentration of bacteria within a test sample. (4) Conclusions: This easy-to-use device, which employs MTT-PMS reagents, can be regarded as a potential and portable tool for rapid water quality determination.
Highlights
Water is an indispensable natural resource for sustaining human life
(3) Results: Compared with traditional methods, our device is simple to operate and can provide rapid (i.e., 5 min) semi-quantitative results regarding the concentration of bacteria within a test sample
Clean water is an important factor for economic development and food production [1]
Summary
Clean water is an important factor for economic development and food production [1]. The indicator bacteria commonly used to monitor water quality are coliforms, Escherichia coli and Pseudomonas aeruginosa [3]. E. coli is a member of the fecal coliform group and is considered to be an important water quality detection indicator [4]. Food and water are frequently contaminated with E. coli from human or animal waste that can survive for a long time in a changing environment [5]. For all of these reasons, measurement of E. coli content in water is both a viable and extremely valuable approach for determining water quality
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