Abstract
The U.S. National Institute of Standards and Technology (NIST) sequenced 1036 human DNA samples from four United States population groups (African American, Asian, Hispanic, and Caucasian) using the ForenSeq DNA Signature Prep Kit with Primer Mix B (DPMB) on a MiSeq FGx instrument. In addition to STR markers, DPMB includes amplification primers for single nucleotide polymorphisms (SNPs) used for individual identification (iiSNPs, n = 94), ancestry inference (aiSNPs, n = 56), and phenotype prediction (piSNPs, n = 22). Resulting sequencing coverage information was interpreted for the 94 iiSNP markers. Here we present performance characteristics of the ForenSeq DNA Signature Prep Kit in the population studied.
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