Abstract
Hairy root cultures are considered as a valuable source of bioactive phytoconstituents with expanding applicability for their production. In the present study, hairy root cultures of Polyscias filicifolia (Araliaceae), a traditional Southeast Asian medicinal plant, were established. The transformation with Agrobacterium rhizogenes ATCC 15834 allowed to obtain 15 root lines. The K-1 line, demonstrating the highest growth capabilities, was subjected to further investigations. To enhance the biosynthetic potential of hairy roots, methyl jasmonate elicitation approach was applied (MeJA; at different doses and exposure time), with subsequent transfer of elicited roots to control medium. This strategy resulted in chlorogenic acid production up to 1.59 mg/g dry weight. HPLC-PDA-ESI-MS analysis demonstrated variation in extracts composition and allowed to identify different caffeic and ferulic acid derivatives. Next, cytotoxic, antigenotoxic, and anti-photogenotoxic properties of hairy roots extracts were determined. None of the tested extracts were cytotoxic. In addition, they demonstrated significant antigenotoxic activity with the highest protective potential; up to 52% and 49% of inhibition of induction ratio (IR) induced by the 2-aminoanthracene was revealed for extracts derived from hairy roots elicited for 3 days with 50 µM MeJA and roots elicited for 7 days with 100 µM MeJA and then transferred for 30 days to control medium, respectively. These same extracts exhibited the highest anti-photogenotoxic potential, up to 36% of inhibition of chloropromazine-induced genotoxicity.
Highlights
IntroductionIntroduction published maps and institutional affilPlant biotechnological methods offer the possibility for production of a wide array of various bioactive compounds for pharmaceutical, as well as food and cosmetic industries in a manner independent of environmental conditions, at the same time ensuring protection of biodiversity of natural resources [1]
Introduction published maps and institutional affilPlant biotechnological methods offer the possibility for production of a wide array of various bioactive compounds for pharmaceutical, as well as food and cosmetic industries in a manner independent of environmental conditions, at the same time ensuring protection of biodiversity of natural resources [1]
This study reports, for the first time, the method of establishing Polyscias filicifolia hairy root cultures and investigation of changes in their phytochemical profile affected by elicitation
Summary
Introduction published maps and institutional affilPlant biotechnological methods offer the possibility for production of a wide array of various bioactive compounds for pharmaceutical, as well as food and cosmetic industries in a manner independent of environmental conditions, at the same time ensuring protection of biodiversity of natural resources [1]. Among various systems of cultivated in vitro cell and organs, hairy root cultures are recognized as a reasonable system for efficient production of complex specific metabolites, as they are capable of producing the same profile of metabolites as a native plant, or even de novo synthesizing biomolecules [2,3,4,5]. Many methods are used in plant biotechnology to increase the efficiency of the production of specific metabolites in cells, tissues, and organs of plants grown in in vitro cultures. In response to elicitor stress, the production of plant-specific metabolites increases.
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