Abstract
Recent analytical techniques can be applied to separate and quantify polyphenols in extracts of propolis used in nutraceutical preparations and to determine the typical ‚fingerprints’ of propolis for the reliable identification of a large number of polyphenolic components. Our laboratory has developed a multi-analytical approach for the identification and chemical characterization of extracts of propolis coming from different countries of origin including spectrophotometric procedures such as HPLCUV- ESI-MS and 1H- and 13C-NMR for an accurate assessment of the contents of these compounds in extract samples. The results show that HPLC-UV-ESI-MS and NMR are useful tools for the quantification of bioactive substances in the different types of propolis and for the chemical classification of propolis. These techniques may provide alternatives to classical analytical phytochemistry to screen commercial preparations of propolisand to evaluate specific nutraceutical benefits. The availability and use of efficient analytical methodologies to document the quantitative composition of bioactive molecules in plant extracts is essential for consumer protection and to comply with the rigorous rules of recent legislation.
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