Abstract

The composition and content of polyphenols in fruit pericarp and pulp  of 12 Apple landraces grown  of Heilongjiang Province were determined by ultra-high performance liquid chromatography (UPLC), and the differences in the composition and content characteristics of polyphenols in different varieties were studied, and the main factor analysis and cluster analysis were carried out. The results showed that 5 kinds of 20 kinds of polyphenols were detected in 12 Apple landraces , the main phenolic substances in pericarp were epicatechin and catechin, and the main phenolic substances in pulp were chlorogenic acid, catechin and procyanidin. There were significant differences in the composition and content of polyphenols among different varieties, the smallest coefficient of variation was quercetin 3-glucoside (37.48%), and the largest coefficient of variation was rutin (267.18%). It was found that the content of proanthocyanidins in pericarp and pulp was higher by principal component analysis. Based on the content of polyphenol components, it was found that the results of pulp clustering were more consistent with the field phenotypic identification results and kinship. The results provide data support and theoretical basis for processing and utilization of phenolic substances and breeding of local varieties in Heilongjiang province.

Highlights

  • Heilongjiang Province, located in the northeast of China, has a temperate continental monsoon climate and is the province with the lowest average temperature in China

  • The results showed that 5 kinds of 20 kinds of polyphenols were detected in 12 Apple landraces, the main phenolic substances in pericarp were epicatechin and catechin, and the main phenolic substances in pulp were chlorogenic acid, catechin and procyanidin

  • The results provide data support and theoretical basis for processing and utilization of phenolic substances and breeding of local varieties in Heilongjiang province

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Summary

Introduction

Heilongjiang Province, located in the northeast of China, has a temperate continental monsoon climate and is the province with the lowest average temperature in China. Over the past 60 years, Heilongjiang Province has bred a number of apple varieties with good fruit shape, high quality, excellent storage capacity and strong cold resistance through introduction, selection, breeding and inspection, but the specific components of these varieties are still unclear. 12 apple landraces grown in Heilongjiang Province were used as test materials, and the components and content of pericarp and pulp were deeply studied and analyzed by high performance liquid chromatography, so as to reveal the differential characteristics of polyphenolic content in apple fruits during the ripening period in cold regions, and provide data support and theoretical basis for the processing and utilization of phenolic substances and the further breeding of apple varieties

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