Abstract
Polymorphisms of the CSN1S1 Gene and its Protein Variants in River and Swamp Buffalo (Bubalus bubalis)
Highlights
This study was aimed to evaluate the effects of feeding frequency on growth performance, antioxidant status and immunity of juvenile red swamp crayfish Procambarus clarkia
white spot syndrome virus (WSSV) challenge experiment After the initial sampling, 10 crayfish with similar body weight obtained from each tank were moved to a new plastic tank (1.0 m×0.4 m×0.4 m, height of water 0.1 m) and allowed to acclimate for 2 days before the challenge
The weight gain rate (WGR) of crayfish fed 3 times/ day was significantly higher than crayfish fed 1, 4 and 5 times/day, the Feed conversion ratio (FCR) of crayfish fed 2 and 3 times/day were significantly lower than those fed 1 and 5 times/ day (P < 0.05)
Summary
Body weight per day, and feed amount was adjusted once every two weeks based on its growth rate to ensure that all Crayfish and test diets feed was eaten. H, water height: 0.2 m) for 2 weeks to acclimate to the The feeding frequency and time followed in different experimental conditions. Online First Article were fed with test diet once per day (19:00) at a feeding rate of 4% whole body weight. WSSV challenge experiment After the initial sampling, 10 crayfish with similar body weight obtained from each tank were moved to a new plastic tank (1.0 m×0.4 m×0.4 m, height of water 0.1 m) and allowed to acclimate for 2 days before the challenge. Results were expressed by mean ± SEM of the replicate groups (3 tanks per treatment; n= 3)
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