Abstract
In the present method appropriate N-protected-5′-0-(4,4′-dimethoxytrityl)-2′-deoxynucleosides are phosphorylated with the reagent 2-chlorophenyl-0,0-bis(1-benzotriazolyl)-phosphate and the 3′-phosphorylated intermediates thus obtained are used directly to elongate the polymer support Kieselgur-polydimethylacrylamide. According to this procedure, we prepared two DNA fragments: one decamer and a heptadecamer.
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