Abstract
Using high resolution molecular technique of atomic force microscopy, we address the extracellular polymer production of Adriatic diatom Cylindrotheca closterium analyzed at the single cell level and the supramolecular organization of gel phase isolated from the Northern Adriatic macroaggregates. Our results revealed that extracellular polysaccharides freshly produced by marine diatoms can self-assemble directly to form gel network characteristics of the macroscopic gel phase in the natural aquatorium. Based on the experiments performed with isolated polysaccharide fractions of C. closterium and of macroaggregates gel phase, we demonstrated that the polysaccharide self-assembly into gel network can proceed independent of any bacterial mediation or interaction with inorganic particles.
Highlights
The most intensive primary production and DOM transformation in the Mediterranean basin takes place in the northern part of the Adriatic Sea [1]
Those questions can be resolved using high resolution imaging technique of atomic force microscopy (AFM) to visualize polymer networks produced by marine diatom in the culture and networks of macroscopic gel phase formed during the “mucilage” episodes in the northern Adriatic basin
Polysaccharide fraction isolated from cell culture and from macroscopic gel phase will be used to compare the capacities for network formation without the influence of bacterial action and without presence of mineral particles as nucleation sites
Summary
The most intensive primary production and DOM transformation in the Mediterranean basin takes place in the northern part of the Adriatic Sea [1]. (iii) photosynthetically produced polymers adsorb on mineral nanoparticles that act as nucleation centers for the gelation to proceed Those questions can be resolved using high resolution imaging technique of atomic force microscopy (AFM) to visualize polymer networks produced by marine diatom in the culture and networks of macroscopic gel phase formed during the “mucilage” episodes in the northern Adriatic basin. We address the extracellular polymer production of C. closterium isolated from the northern Adriatic Sea. Polysaccharide fraction isolated from cell culture and from macroscopic gel phase will be used to compare the capacities for network formation without the influence of bacterial action and without presence of mineral particles as nucleation sites
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