Polygenic powdery mildew disease resistance in Arabidopsis thaliana: quantitative trait analysis of the accession Warschau‐1

Abstract

To further the understanding of the natural genetic diversity for disease resistance to powdery mildew (Erysiphe cichoracearum) in Arabidopsis thaliana, quantitative trait loci analysis was undertaken on recombinant inbred lines derived from a cross between the resistant accession Warschau‐1 and the susceptible Columbia‐0. Powdery mildew grew less well on Warschau‐1, but the resistance was not associated with a specific block in the infection sequence. Two potential powdery mildew disease‐resistance loci were identified and mapped, one with a major effect and one with a minor effect on disease resistance. The two loci acted in an additive manner to confer resistance, and together they explained 65% of the variation in resistance. In addition, the major powdery mildew disease‐resistance locus was genetically mapped to the bottom of chromosome III, a region containing the powdery mildew resistance loci RPW7, RPW8 and RPW10. Unlike resistance mediated by the RPW8 locus in the accession Moscow‐1, resistance in Warschau‐1 was not correlated with the hypersensitive response, highlighting the influence of genetic background or environmental factors on the expression of disease resistance. Together with the powdery mildew resistance loci described in other studies, these results suggest that A. thaliana is a useful source of natural powdery mildew disease resistance, which potentially can be utilized in fundamental studies and as a tool for applied studies.