Abstract
MPEG-PLE was prepared by modification of PLE with 1 and characterized by gel electrophoresis, size exclusion chromatography, anion exchange chromatography and photon correlation spectroscopy. It retains the activity of native PLE in the enantioselective hydrolysis of prochiral diesters 2, 4, 6 and 8 to the monoesters 3, 5, 7 and 9, respectively, in water. MPEG-PLE is soluble and active in organic solvents. The MPEG-PLE catalyzed acylation of prochiral diols 10 and 11 with vinyl acetate in toluene leads to monoacetates ent- 5 and 9, respectively.
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