Polyacrylonitrile (PAN) nanofibres spun with copper nanoparticles: an anti-Escherichia coli membrane for water treatment.

Abstract

Copper nanoparticles (CNPs) were mixed with polyacrylonitrile (PAN) and electrospun into nanofibres (CuPAN nanofibres). PAN nanofibres containing 1.0, 3.0 and 5.0% copper (w/v) displayed beads-on-string morphology with protrusions of copper particles. The diameter of the CuPAN nanofibres differed according to the copper content, ranging from 386nm (1.0%, w/v, copper) to 922nm (5.0%, w/v, copper). No chemical interaction of copper with PAN was observed when studied with X-ray diffraction, ATR-FTIR (attenuated total reflection-Fourier transform infrared) spectroscopy and TGA (thermogravimetric analysis). None of the CuPAN nanofibres showed signs of degradation after 7days in water. Bacteria suspended in random mobility buffer and filtered through a 3% CuPAN nanofibre membrane (25mm diameter, 75-80μm thickness), at a filtration rate of 20mlmin-1, reduced the cell numbers of enterotoxigenic Escherichia coli (ETEC) from 3.3 × to 2.1 × 106cfuml-1 and methicillin-resistant Staphylococcus aureus (MRSA) from 1.2 × 10 to 1.3 × 103cfuml-1. Membranes produced with 1.0, 3.0 and 5.0% (w/v) CuPAN inhibited the growth of enteroaggregative E. coli (EAEC), enterohemorrhagic E. coli (EHEC), enteroinvasive E. coli (EIEC), enteropathogenic E. coli (EPEC), ETEC and MRSA, as shown with LIVE/DEAD™ BacLight™ staining. Real-time bactericidal activity of CuPAN membranes was recorded by staining the cells with SYTO 9 and PI, followed by flow cytometry. Filter membranes made from CuPAN fibres may be used to reduce pathogenic E. coli cell numbers in potable water.