Abstract
The adsorption characteristics of several polyoxyethylene-polyoxypropylene (POE-POP) block copolymers (Synperonics) were studied on both polystyrene microspheres and small unilamellar vesicles (SUVs) composed of egg phosphatidylcholine (egg PC). Photon correlation spectroscopy (PCS) was used to monitor the increase in hydrodynamic radius following polymer sorption. Apparent adsorbed layer thickness (δ) of all POE-POP block copolymers was shown to be Langmurian. This δ increased non-linearly with the molecular weight of the polymer. Adsorption of Synperonic F108 occurred rapidly onto latex particles with no subsequent change of hydrodynamic radius on storage. For SUVs, δ was significantly less ( P < 0.05) than that measured for the same polymer with polystyrene microspheres, which may indicate a degree of bilayer penetration by the polymers. The efflux rate of entrapped 6-carboxyfluorescein (6-CF) was considerably lower for SUVs prepared from egg PC and cholesterol (1 : 1 molar ratio) than for those prepared from egg PC alone when suspended in Synperonic F108. Retention of 6-CF was higher for multilamellar vesicles (MLVs) than for SUVs when incubated in the same polymer solution.
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