Abstract
Polarisation imaging has the potential to provide enhanced contrast based on variations in the optical properties, such as scattering or birefringence, of the tissue of interest. Examining the signal at different wavebands in the visible spectrum also allows interrogation of different depths and structures. A stereo endoscope has been adapted to allow snapshot acquisition of orthogonal linear polarisation images to generate difference of linear polarisation images. These images are acquired in three narrow bands using a triple-bandpass filter and pair of colour cameras. The first in vivo results, acquired during a surgical procedure on a porcine subject, are presented that show wavelength dependent variations in vessel visibility and an increase in contrast under polarised detection.
Highlights
Analysis and manipulation of the polarisation properties of light reflected by tissue from a polarised input beam have been used to perform different types of biomedical measurements including sub-epidermal skin imaging [1], colon cancer margin detection [2], characterisation of skin lesions [3], and visualisation of tissues exhibiting dichroic or birefringent properties [4, 5]
This paper has presented an endoscopic system capable of acquiring co- and cross-polarised light images of tissue during surgery using a stereo endoscope modified with a polarising filter tip
Depth gating of photons is achieved by generating differential Pol images, which are free from motion artefacts due to the synchronous detection of the imaging channels
Summary
Analysis and manipulation of the polarisation properties of light reflected by tissue from a polarised input beam have been used to perform different types of biomedical measurements including sub-epidermal skin imaging [1], colon cancer margin detection [2], characterisation of skin lesions [3], and visualisation of tissues exhibiting dichroic or birefringent properties [4, 5]. It has been suggested as a means of identifying abnormal tissue during surgery in conditions such as endometriosis [5] where endometrial tissue develops outside the uterus as lesions. Some current widely-used standard endoscopes contain birefringent optics, which alter the polarisation properties of light in a way that may not be reversible [9]
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