Abstract
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Highlights
The growth and survival of single-celled organisms relies on their ability to adapt to rapidly changing environmental conditions
To determine the contribution of individual cell wall enzymes to pH-dependent growth, we cultured strains harboring deletions in genes encoding each of three class A penicillin binding proteins (PBPs), six LD-transpeptidases, five carboxypeptidases, four amidases, nine lytic glycosyltransferases, and six endopeptidases to mid-exponential phase (OD600 ~0.2–0.6) in buffered lysogeny broth (LB) media sub-cultured them into fresh LB buffered to pH 4.8, 6.9, or 8.2 for growth rate analysis
Preliminary hits were identified by a significant (>5%) decrease in early exponential phase (OD6000.005–0.1) mass doublings per hour (DPH) in at least one pH condition compared to the parental strain
Summary
The growth and survival of single-celled organisms relies on their ability to adapt to rapidly changing environmental conditions. One model to account for the apparent redundancy of periplasmic cell wall proteins is that enzymes within a given class may be specialists for distinct environmental niches, thereby allowing bacteria to cope with the diverse chemical and physical properties that might affect protein stability and function in this compartment (Pazos et al, 2017). In support of this hypothesis, several groups have identified cell wall enzymes that have increased activity in acidic media. We further demonstrate that synthase specialization has consequences for intrinsic resistance to b-lactam antibiotics in nonstandard growth conditions
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