Plastic effect of soiled bedding on the structure of synapses in rat accessory olfactory bulb.

Abstract

The effects of exposure to soiled bedding on synaptic morphology in the accessory olfactory bulb (AOB) were examined in adult male rats. Forty-day-old male rats were isolated. One group was exposed to bedding soiled by male and female rats (EC). Another group was exposed only to male-soiled bedding (SC). A third group was exposed to clean bedding (IC). After 2 months, the animals were sacrificed for electron microscopy. The size and the numerical density of synapses were measured in the glomerulus and the granule cell layer. In the glomerulus, the mean size of the synapses was significantly greater in the EC than in the IC group, whereas that in the SC group appeared to be intermediate between those in the EC and the IC groups but was not significantly different from those in the EC and the IC groups. There was no statistically significant difference in the density of synapses among the three groups. Synapses in the granule cell layer are classified into two types: 1) perforated synapses, which are characterized by discontinuities in their postsynaptic thickenings, and 2) nonperforated synapses. The mean size of perforated synapses was significantly greater in the EC than in the IC and SC groups; however, no statistically significant difference was observed in the size of nonperforated synapses among the three groups. Moreover, no statistically significant difference was observed in the numerical densities of either perforated or nonperforated synapses among the three groups. These results suggested that exposure to a more complex soiled bedding environment (i.e., to bedding soiled by both male and female rats) can induce greater structural changes of the synapses in the AOB of male adult rats.