Abstract
Metal-enhanced fluorescence of molecular probes in plasmonic nanostructures offers highly sensitive chemical and biomedical analyses, but a comprehensive theory of the phenomenon is far from being complete. In this study, a systematic theoretical analysis is provided for overall luminescence enhancement/quenching for fluorophores near silver spherical nanoparticles. The approach accounts for local intensity enhancement, radiative and nonradiative rates modification, light polarization, molecule position, and its dipole moment orientation. Numerical modeling has been performed for fluorescein-based labels (e.g., Alexa Fluor 488) widely used in biomedical studies and development. The maximal enhancement exceeding 50 times is predicted for nanoparticle diameter 50 nm, the optimal excitation wavelength being 370 nm. For long-wave excitation, bigger particles are more efficient. The experiments with a fluorescein isothiocyanate conjugate of bovine serum albumin confirmed theoretical predictions. The results pr...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
