Abstract
Ultrasound was used to free Plasmodium berghei from host erythrocytes. Debris formation, parasite damage, and red cell-membrane entrapment of parasites were minimized by using a continuous-flow system of sonication. The free parasites were harvested by differential centrifugation, and low-power thin-section electron microscopy showed the effectiveness of the procedure. The free parasites were used as antigens in complement-fixation tests, and they reacted well with sera from rats recovering from Plasmodium berghei infection.
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