Abstract
The use of in vitro membrane vesicles is attractive because of possible applications in therapies. Here we aimed to compare the stability and functionality of plasma membrane vesicles extracted from control and salt-treated broccoli. The impact of the amount of aquaporins was related to plasma membrane osmotic water permeability and the stability of protein secondary structure. Here, we describe for first time an increase in plant aquaporins acetylation under high salinity. Higher osmotic water permeability in NaCl vesicles has been related to higher acetylation, upregulation of aquaporins, and a more stable environment to thermal denaturation. Based on our findings, we propose that aquaporins play an important role in vesicle stability.
Highlights
In recent years, high importance has been given to membrane vesicles due to the potential application as carriers in therapies
In other experiments, results have shown that broccoli plants under high salinity stress (80 mM NaCl) markedly modify the plasma membrane lipid composition and induce a significant accumulation of PIP1 and PIP2 homologs
When broccoli plants were exposed to salinity stress (100 mM) for 15 d, the transcript level of BoPIP2;2 and BoPIP2;3 was increased in roots of the Naxos cultivar [17]
Summary
High importance has been given to membrane vesicles due to the potential application as carriers in therapies. This vesicles coming from plasma membrane, called extracellular vesicles (EVs) has been reported as fundamental for intercellular communication [1]. In addition to being an important crop with high economic returns, Brassica crops might represent a source of agronomical by-products. The marketable production of broccoli represents only 25% of its total biomass, the remaining by-product 75% (about 500.000 tons) consists of leaves and stems without commercial or economic value, involving additional agricultural waste management costs for farmers. We found that chemical changes appeared in the plasma membrane of Brassica leaves
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