Abstract
The objectives of this study were to investigate the plasma E 1S and E 2β profiles during pregnancy and their relationship with the relaxation of sacrosciatic ligament in Holstein–Friesian cattle ( n = 37) and then to predict the calving time on the basis of E 1S and E 2β profiles and relaxation of the ligament. Blood samples were collected at 4 weeks intervals from days 100 to 190, at 2 weeks intervals from days 190 to 250, every week from days 250 to 270 and thereafter every day from day 270 of gestation until the day after calving. The relaxation in the ligament was measured by using two scales as a distance at a schedule similar to blood sampling plus 5 days postpartum. One scale was kept firm exactly parallel to the ligament between the sacrum and the tuber ischii and other scale was erected perpendicularly to the first scale with the bottom just touching the ligament and the depth was measured in the second scale from the point where it touched the ligament to the point where it touched the first scale. Plasma samples were analyzed for E 1S and E 2β by enzyme immunoassay. E 1S concentration was low at day 100 (0.8 ± 0.3 ng/ml), then increased progressively and drastically to reach the level of 28.4 ± 3.6 ng/ml on the day before calving and declined significantly ( p < 0.05) at 9.5 ± 3.1 ng/ml within 1 day postpartum. There was a gradual increase in concentration of E 2β from day 100 of gestation (0.1 ± 0 ng/ml) until day 4 prepartum (0.6 ± 0 ng/ml). Thereafter, it increased drastically and reached the peak level of 1.0 ± 0.1 ng/ml ( p < 0.05) on the day before calving and declined markedly at 0.4 ± 0.1 ng/ml within 1 day postpartum ( p < 0.05). Corresponding to E 1S and E 2β concentrations, a gradual increase in the relaxation of the ligament was observed from day 100 of gestation (8 ± 1 mm) until day 2 prepartum (24 ± 2 mm). Thereafter, it showed a significant increase ( p < 0.05) within 1 day before calving (31 ± 2 mm) and almost no difference between day 1 prepartum and day 1 postpartum. A marked decrease ( p < 0.05) was observed thereafter until day 3 postpartum (10 ± 2 mm) and no significant change between days 3 and 4 as well as 4 and 5 postpartum. The increment of E 2β by ≥0.20 ng/ml from the preceding day concentration was 85.2% accurate for predicting calving within 24 h in many of the cows (23 of 37) in the herd. The increment in ligament relaxation measurement by ≥5 mm from the preceding day measurement was the most efficacious to predict calving within 24 h with the highest accuracy (93.9%) in high proportions of cows (31 of 37) in the herd. In conclusion, plasma E 1S and E 2β concentrations and relaxation of sacrosciatic ligament increased gradually as gestation advanced and reached the peak level on the day before calving. The relaxation in the ligament corresponded well to plasma E 2β concentrations. Prediction of calving was possible by E 2β profile and relaxation in the ligament but not by E 1S profile. The increment in ligament measurement by ≥5 mm from the preceding day measurement was the most useful and accurate in predicting calving within 24 h. It is economical and easily applicable in the field condition.
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