Abstract
This report describes a reproducible plaque assay for lentogenic strains of Newcastle disease in primary cultures of chick embryo fibroblasts (CEF). The conditions required for this test are: 1) a suitable agar overlay medium; 2) high levels of Mg++; 3) use of solidifying agents with low-sulfated polysaccharides; and 4) high concentration of diethylaminoethyl (DEAE) dextran. The importance of divalent cations in the attachment of phages to the bacterial host cell was established by Puck and co-workers (5,6,10). More recently, a number of investigators (4,15,16,17,18) have reported the enhancement of rhinovirus and enterovirus plaque formation by increased Mg+ . DEAE dextran has been found to prevent or reverse the inhibitory effects of sulfated polysaccharides from agar (2,4,8,14) and to facilitate the uptake of viral ribonucleic acid (11) or complete virus particles (7) by cells in tissue culture.
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