Abstract
Green callus was obtained from the surface of embryogenic yellow callus which was induced from Allium fistulosum floret tissue on MS agar medium containing 0.5 mg/l 2,4-D and 0.5 mg/l BA. The initiation of green callus was significantly promoted by 0.5–1.0 mg/l BA supplement in culture medium. After the cell clumps developed from green callus were transferred to MS liquid medium supplemented with 0.1 mg/l of 2,4-D, proembryos formed. The proembryos put through a stainless sieve developed into synchronous somatic embryos, and these somatic embryos matured in MS liquid medium either without growth regulators or containing 0.1 mg/l of zeatin and 0.1 mg/l of ABA. Plantlets developed from mature embryos in MS medium either without growth regulators or containing 0.1 mg/l BA, and they were regenerated to plants in pots.
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