Abstract
Stimulation of multiple shoot-bud formation of soybeans in culture has been accomplished using conditioned cotyledonary node segments. Germination of soybean seeds in the presence of 10–50 μM BAP stimulated varying numbers of buds to form at the cotyledonary node region. Culturing cotyledonary node segments excised from such conditioned seedlings on a medium containing 5–50 μM BAP stimulated multiple shoot-bud formation. Continual bud multiplication was obtained by periodic subculture of the organized tissue mass onto fresh media containing the same BAP concentration. Bud growth was stimulated by transferring cultures from a high BAP concentration of 10–50 μM to a low BAP concentration of 1 μM. Soybean plants were produced by rooting tissue culture shoots on a basal medium without auxin.
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