Abstract
Sexual reproduction plays an essential role in the propagation of Angiosperms. Fertilisation takes place in the embryo sac, which is usually deeply encased in the sporophytic tissues of the ovule. In contrast to animals and primitive plants, the mechanism of egg cell activation in flowering plants has not been discovered fully because of the inaccessibility and complexity of the process of double fertilisation. However, recent advances in plant cell and molecular biology have brought new, powerful technologies to investigate and micromanipulate the reproductive cells of flowering plants including cereal crops. An experimental approach based on various micromanipulation techniques involving in vitro fertilisation (IVF) and microinjection procedures is now available in more and more laboratories. Despite some limitations this offers new possibilities to study cellular and subcellular events preceding or occurring during or after egg cell activation and early embryonic development. Recent achievements in the field of wheat egg cell micromanipulation are presented in this paper.
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